GARS, also known as glycyl-tRNA synthetase, is one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This enzyme is an (alpha) 2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.
Source:Recombinant protein corresponding to aa43-289 from human GARS, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight:~30kD (270aa), confirmed by MALDI-TOF
Applications:Suitable for use in SDS-PAGE. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~85% (SDS-PAGE)
Form：Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.
