A cell-permeable imidazolyl-isoxazolamide that upregulates b-catenin-regulated transcription (CRT; 10-40uM) by disrupting beta-catenin and Axin interaction, thereby preventing b-catenin phosphorylation (Ser33/Ser37/Thr41/Ser45) and proteasomal degradation, without affecting the activities of GSK-3alpha/beta or 18 other kinases (<8.5% inhibition by 10uM SKL2001). Shown to effectively promote osteoblastogenesis in both human and murine mesenchymal cultures (10 to 40uM) as well as suppress MDI- (dexamethasone and insulin) stimulated adipogenesis of murine 3T3-L1 preadipocytes (5 to 30uM).
Formula:C14H14N4O3
Molecular Weight:286.3
Solubility:DMSO (100mg/ml, clear, red solution)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
