A cell-permeable (quinoxalinylmethylidene-D-Tryptophanyl)hydrazide that directly targets Rho GEF binding domain (Kd = 354nM for RhoA), thereby prevents Rho from interacting with its GEFs (including LARG, DBL, LBC, p115 RhoGEF, and PDZ RhoGEF), but not p190RhoGAP, RhoGDI, or its effectors (ROCK, mDia, PKN, Rhoteckin). Shown to inhibit serum-induced RhoA, RhoB, and RhoC, but not Cdc42 or Rac1, activation, MLC & PAK phosphorylation, as well as stress fiber & focal complex formation in serum-starved NIH3T3 cultures (Effective conc. 30uM) in a reversible manner.
Storage and Stability:May be stored at 4°C for short-term only. Store at -20°C under inert atmosphere. Protect from light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
