A cocktail of five protease inhibitors with broad specificity for the inhibition of cysteine, serine, aspartic, and thermolysin-like proteases and aminopeptidases. This cocktail is recommended for purification of proteins containing His-tag sequences. Each vial contains 100mM AEBSF, HCl, 5mM Bestatin, 1.5mM E-64, 2mM Pepstatin A, and 200M of Phosphoramidon, Disodium Salt. One ml is recommended for the inhibition of proteases in 10g of cells. Supplied with a data sheet. Note: 1set=5x1ml.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.