A cell-permeable 3-MB-PP1 analog that is more effective than 3-MB-PP1 in inhibiting SykM442A S505A ASKA- (analog-sensitive kinase alleles) mediated cellular calcium flux (98% vs. 20% inhibition, respectively, by 1uM 2,3-DMB-PP1 or 3-MB-PP1) in Syk MASA-expressing DT40 cells. Also shown to selectively inhibit L159G ASKA, but not wt, PDK1-dependent GSK3&alpha Ser21 and S6 Ser235/236 phosphorylation upon IGF1 stimulation in mES cells.
Formula:C18H23N5
Molecular Weight:309.4
Solubility:DMSO (100mg/ml)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
![PP1 Analog V, 2,3-DMB-PP1 (4-Amino-1-tert-butyl-3-(2,3-dimethylbenzyl)pyrazolo[3,4-d]pyrimidine, 1-tert-Butyl-3-(2,3-dimethylbenzyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine, Syk ASKA Inhibitor II, PDK1 ASKA Inhibitor) 10mg](https://aimg.as-1.co.jp/c/85/2075/77/85133520.jpg)