Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes; PDE3B may play a role in fat metabolism.
Source:Recombinant protein corresponding to full length human phosphodiesterase 3B, fused to His-tag at the N-terminal domain expressed in S. frugiperda insect cells using a baculovirus expression system.
Biological Activity:≥0.5units/ul
Unit Definition:One unit is defined as the amount of enzyme that will convert 1 pmole cAMP to AMP per min at pH 7.5, 30°C.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:10u
Source Antigen:Recombinant, S. frugiperda insect cells using a baculovirus expression system
Grade:Purified
Purity:Partially Purified
Form:Supplied as a liquid in 10mM Tris, pH 8.3, 150mM sodium chloride, 0.1% NP-40.