A 2’,3’-dialdehyde derivative of ATP that is commonly used to affinity label nucleotide sites in enzymes. Acts as a specific, irreversible antagonist of P2Z/P2X7 purinergic receptors in J774 mouse macrophages. Does not affect P2g receptors. Inhibits inflammatory pain in arthritic rat model by blocking ATP action on P2X7 receptor in nerve terminals. Also shown to irreversibly block ATP-induced Ca2+ influx in lymphocytes (˜100uM). O-ATP-treated mice display better preservation and lower rate of rejection of pancreatic islet grafts with reduced Th1 transcripts.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
