A cell-permeable, thiazolidinedione (TZD) that acts as an inhibitor of MitoNEET (IC50=0.9uM) in competition with rosiglitazone and pioglitazone, in liver mitochondrial suspensions. In pharmacological studies, it was found to alter isolated mitochondrial complex I respiration with an IC50 of 2.4uM and inhibited State III respiration up to 45%. Furthermore, this compound (6-25uM) is shown to dose-dependently reduce lactate-dehydrogenase release in SH-SY5Y cultures, leading to a 40% decrease in rotenone-induced cell-death.
Formula:C18H25NO3S
Molecular Weight:335.46
Solubility:DMSO (100mg/ml, clear, pale yellowish solution)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
