A cell permeable naphthalene derivative that promotes the translocation of ATF2 from nucleus to mitochondria via PKCe that results in mitochondrial membrane leakage and cell death in melanoma cells (WM793 and UACC903). Diminishes cell motility and anchorage-free growth in melanoma cells (<90% at 10uM) and reduces their migration in an ATF2-dependent manner. Shown to reduce ATF2 phosphorylation on Thr52, 69, and 71. However, it does not affect the activity of Erk1/2, Akt, pan-PKC. Cells expressing ATF2T52E do not exhibit any loss of mitochondrial membrane potential. Does not induce apoptosis in normal melanocytes even at high concentrations (~20 mM) and has no effect on the localization or translocation of p53, STAT3, or b-catenin.
Solubility:Warm DMSO
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
