A cell-permeable, stable dichlorosalicylaldehyde Schiff's base that acts as a potent, selective inhibitor of Mia40/Erv1 redox-mediated import pathway (IC50 = 700nM, 900nM, and 1.4uM for ALR, Erv1, and Erv2, respectively). Significantly reduces the import of CX9 proteins, Erv1, Tim23, and ADP/ATP carrier (AAC). However, it does not affect mitochondrial membrane integrity as evidenced by the lack of aconitase, AAC, Tim54, Mia40, and cytochrome c release. Has no effect on protein disulfide isomerase, flavin adenine dinucleotide, and succinate dehydrogenase activities and does not disrupt mitochondrial net work or reduce viability of cells even at high concentrations (~100uM in HeLa and HEK293 cells). Reported to specifically cause cytochrome c release, activate caspase-3, and induce apoptosis in human embryonic stem cells (~20uM), but not in differentiated cells. Reversibly impairs cardiac development and reduces heart rate in zebra fish that is attributed to mitochondrial dysfunction.
Molecular Formula:C₁₉H₁₄Cl₂N₂O
Primary Target:Erv1
Molar Mass:357.2
Solubility:DMSO
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
