A symmetrical bis-(amino, hydroxynaphthalenedisulfonate) compound that antagonizes macrophage migration inhibitory factor against receptor binding (IC50=810nM against hMIF and CD74 interaction) and is more potent than ISO-1 in suppressing MIF-induced cytokines and MMP-3 productions in human fibroblasts cultures (IC50= <0.7uM vs >5uM). Unlike MIF Antagonists I-IV, p425 targets an area at the interface of two MIF trimers, distal to the active site.
Formula:C34H24N6Na4O16S4
Molecular Weight:992.8
Solubility:H2O (25mg/ml)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
