A cell-permeable phenylpropenoate compound that inhibits the proliferation of colon cancer cell lines exhibiting high levels of KLF5 (IC50=1.6uM in DLD-1 cells). Downregulates the expression of early growth response protein 1 (EGR1), which modulates KLF5 expression at transcription level. Shown to activate the EGFR/ERK pathway by stimulating phosphorylation of EGFR-Tyr1068 and ERK1/2-Thr202/Tyr204. Also, up-regulates the phosphorylation of p38 MAPK-Thr180/Tyr182.
Formula:C16H18N2O7S
Molecular Weight:382.4
Solubility:DMSO (50mg/ml)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
