A cell-permeable, benzoimidazole, renal outer medullary potassium channel (ROMK) blocker that selectively inhibits inward rectifier potassium channel, Kir1K.1 (IC50=240nM), in HEK-293 cells. At 10uM, it displays no effect on Kir2.1, Kir2.3, Kir4.1, and Kir7.1, with 150-fold selectivity over Kir6.2. In addition, it exhibits a modest off-target effect on the GABAA receptor (IC50=6.2uM) among a panel of cardiac and central nervous system ion channels and receptors.
Formula:C16H12N6O5
Molecular Weight:368.3
Solubility:DMSO (100mg/ml, clear, dark brown solution)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
