A cell-permeable biphenylcarbamoylbenzoate compound that potently induces the differentiation of human mesenchymal stem cells (hMSCs) into chondrocytes (EC500nM). Reversibly binds to the FC-1 fragment of filamin A and disrupts its association with CBFB leading to nuclear localization of CBFB and binding to runt-related transcription factor (RUNX) to regulate chondrogenesis. Also reported to inhibit cytokine-induced release of nitric oxide (NO) and glycosaminoglycan (GAG). Exhibits chondroprotective properties and promotes cartilage repair in murine osteoarthritis models. Shown to up-regulate the levels of TIMP1, collagen, and aggrecan thereby blocking further matrix breaown without affecting the activities of MMP-3, MMP-13, and aggrecanase in primary chondrocytes or hMSCs.
Molecular Formula:C₂₀H₁₅NO₃
Molecular Weight:317.3
Solubility:DMSO (100mg/ml)
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
