A methyl ester prodrug of in vitro active N-hydroxybutenoic acid compound that is shown to preferentially induce hypermethylation of histone proteins (EC50 for H3K4me3 and H3K9me3=10.3 and 8.6uM in KYSE150 cells, and 6.7 and 6.3uM in MCF7 cells, respectively), and arrests cell growth. Acts as a selective inhibitor of Jumonji domain-containing histone demethylases (JHDMs) over prolyl hydroxylases (PHDs), and prevents myogenesis through inhibition of H3K27 demethylase UTX in C2C12 cells at 2uM.
Formula:C₂₈H₃₁N₃O6
Solubility:DMSO
Molecular Weight:505.6
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
