A cell-permeable 3,4-diaminoethylbenzoate-based antioxidant that effectively prevents Glutamate- (5mM; 3h) induced neurotoxicity in cultured rat OHSC (by 56%, 65%, and 71%, respectively, in CA1, CA3, and Dentrate gyrus; [Fer-1] = 2uM, added 0.5h prior to Glutamate) and Erastin- (50uM; 24h) induced HT-1080 death (EC50 = 60nM) by blocking cytosolic and lipid ROS accumulation (by >80% at 0.5uM in 10uM Erastin-treated HT-1080 cells). Fer-1 does not prevent H2O2- induced necrosis, STS-induced apoptosis, or Iron-independent oxidative death by Rotenone.
Solubility:DMSO
Primary Target:Free radical scavenger
Molar Mass:262.4
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
