TAME is a potent ubiquitin ligase inhibitor that specifically blocks Anaphase-Promoting Complex (APC) activation by directly binding to APC and disrupts the IR-tail-dependent interactions between APC and its activator proteins Cdc20 or Cdh1. It stabilizes APC substrates and is shown to inhibit cyclin proteolysis in mitotic Xenopus egg extract with an IC50=12uM.
Synonyms:TAME hydrochloride, APC/C Inhibitor I, APC/C-Cdc20 Interaction Inhibitor I, Cyclosome Inhibitor I, N2-[(4-methylphenyl)sulfonyl]-L-arginine, methyl ester, monohydrochloride
Molecular Formula:C₁₄H₂₂N₄O₄S · HCl
Molecular Weight:378.87
Solubility:DMSO (50mg/ml)
Purity:≥95% (HPLC)
Appearance:White solid
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
