Fibroblast Growth Factor 15 (FGF15), BioAssay™ ELISA Kit (Mouse) is a competitive inhibition immunoassay for in vitro quantitative measurement of FGF15 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range:1.23-100pg/ml
Sensitivity:0.51pg/ml
Intra-Assay CV:<10%
Inter-Assay CV:<12%
Kit Components:*216097A: Microtiter Strips, 1x96 wells *216097B: Standard, 2x1 vial216097C: Standard Diluent, 1x20ml*216097D: Detection Reagent A (green), 1x1 vial*216097E: Detection Reagent B (red), 1x120ul216097F: Assay Diluent A, 1x12ml216097G: Assay Diluent B, 1x12ml216097H: TMB Substrate, 1x9ml*216097J: Positive Control, 46.85pg/ml, 1x1vial 216097K: Stop Solution, 1x6ml216097L: Wash Buffer, 30X, 1x20ml216097M: Detection Reagent A Diluent for 216097D, 1x300ul, 1x300ul
Precaution:The Stop Solution (216097K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability:Store *216097A, *216097B, *216097D, *216097E and *216097J at -20°C. After reconstitution of *216097J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary:1. Prepare all reagents, samples and standards
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C
5. Aspirate and wash 5 times
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50ul Stop Solution. Read at 450nm immediately.
