The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Source:Recombinant protein corresponding to full length active human MEK1, fused to N-terminal GST and C-terminal, His6-tagged, expressed in E. coli.
Molecular Weight:~71kD
Specific Activity:~10883U/mg
Unit Definition:one unit of MEK1 activity is defined as the amount of MEK1 which activates 1uM unactive MAPK2 by 1 unit per minute at 30°C using 100uM ATP. One unit of MAPK2 activity is defined as 1nmol phosphateincorporated into 0.33mg/ml MBP per minute at 30°C with a final ATP concentration of 100uM.
Applications:Suitable for use in Kinase Assay. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~90.5% (SDS-PAGE and Coomassie blue staining)
Form：Supplied as a frozen liquid in 50mM Tris/HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
