Clostridium difficile is a gram-positive motile bacterium which is the leading cause of diarrhoea in developed countries. The incidence of disease in humans varies greatly with age, spore density and the administration of antibiotics. Toxin B (TcdB) is a large exotoxin. Its role in disease is less well understood than that of toxin A, though it seems clear that TcdB contributes to disease. Both toxins modifies the Ras superfamily of small GTPases via glycosylation, inactivating them and leading to the disruption of vital signaling pathways in the cell.
Source:Toxin B Toxoid from Clostridium difficile
Inactivation:Toxoid is produced from Toxin B of C. difficile by inactivation with formaldehyde. The resulting toxoid is essentially nontoxic. At 50ug/ml, the toxoid has no detectable cytotoxicity in a cell rounding assay using human foreskin cells when compared to Toxin B. At the highest dose tested (50ug/mouse), mice injected intraperitoneally showed no toxic signs during the seven days of observation.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Clostridium difficile
Grade：Molecular Biology Grade
Form：Supplied as a lyophilized powder in 0.1M histidine, 0.1M sodium chloride, pH 6.5, 5% trehalose. Reconstitute with 100ul sterile ddH2O. Handle Gently. Do not vortex.
