Tetanus toxin is a potent neurotoxin that is taken up by presynaptic terminals at the neuromuscular junction, travels retrogradely within motor neurons and is transported transsynaptically to spinal cord interneurons. Upon entering the motor neuron, tetanus toxin associates with lipid rafts, is incorporated into non-acidified vesicles, sorted in a Rab 5/7 dependent pathway and transported to the cell body. The toxin is trancytosed so that it enters upstream inhibitory neurons where it cleaves VAMP-2, inhibiting neurotransmitter release. Tetanus toxin inhibits neurotransmitter release with great potency and selectivity, cleaving a bond in vesicle-associated membrane protein-2 (VAMP-2) or synaptobrevin-2, thereby preventing the fusion of synaptic vesicles with the presynaptic membrane. Tetanus toxoid is currently used as a vaccine to produce immunity to tetanus, the disease caused by Clostridium tetani. Additionally, tetanus toxoid functions as a carrier of conjugated polysaccharides in the development of vaccines against encapsulated bacterial pathogens.
Tetanus toxin C-fragment consists of the C-terminal portion of the heavy chain of tetanus toxin. Although it is non toxic, it retains the binding and internalization properties associated with intact tetanus toxin. These properties make C-fragment and its conjugates useful in tracing nerve fiber connections in the CNS. In immunodiffusion studies, tetanus toxoid shows immunoprecipitation when reacted against a specific antiserum to tetanus toxoid.
Source:Tetanus Toxoid from formaldehyde inactivated tetanus toxin
Applications:Suitable for use in immunodiffusion. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inactivation:Formaldehyde inactivated. The resulting toxoid is essentially nontoxic. At the highest dose tested (4ug/mouse) there were no deaths, no signs of illness, and no spastic paralysis during seven days of observation.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Tetanus Toxin
Form：Supplied as a lyophilized powder in 100mM histidine, 100mM sodium chloride, pH 7.0, 5% trehalose. Reconstitute with 50ul sterile ddH2O. Handle Gently. Do not vortex.
