There are seven immunologically distinct botulinum neurotoxins, designated types A-G. Each neurotoxin toxin cleaves one of two target proteins critical for synaptic vesicle fusion to the plasma membrane and neurotransmitter release, either SNAP-25 or Synaptobrevin. The latter is also called VAMP-2. Types A, C and E specifically bind and selectively cleave SNAP-25 while types B, D, F and G cleave Synaptobrevin. Type C also cleaves Syntaxin. The enzymatic activity of these toxins can also be measured using the appropriate native substrate and monitoring the generation of the cleaved product on SDS-PAGE gel electrophoresis.
Source:Recombinant protein substrate corresponding to 214aa from C. botulinum SNAP-25, neurotoxins Type A and E, expressed in E. coli.
Molecular Weight:~24.12kD
Activity:SNAP-25 can be used as a substrate to assess the activity of Botulinum Neurotoxin Type A, Botulinum Neurotoxin Type A light chain (LcA) and Botulinum Neurotoxin Type E light chain (LcE). A 100uI reaction of 5uM SNAP-25 (12ug) was completely cleaved within one hour at 37°C in 20mM Tris-HCI, pH 8.0, 50mM NaCI using LcA or LcE. Detection of the cleavage of SNAP-25 by Light Chain E is readily apparent by running a sample of the digestion on a coomassie stained polyacrylamide gel. The cleavage of SNAP-25 by Type A Botulinum Neurotoxin or light chain is not detectable on a gel, due to the small cleavage product, but can be visualized by performing a Western Blot with primary antibodies specific to Botulinum Type A cleaved SNAP-25 (Research and Diagnostic Antibodies, Inc.).
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~90% (Densitometric analysis)
Form：Supplied as a lyophilized powder in 20mM HEPES, pH 7.4, 1.25% lactose. Reconstitute with 265ul sterile ddH2O.
