Lipopolysaccharide (LPS) structures may be generally described as three linked regions. The first structural feature is the lipid component Lipid A, primarily responsible for the effects of endotoxin. Linked to Lipid A is the core antigen or R polysaccharide, a short chain of sugars including 2-keto 3-deoxyoctonate (KDO). The third structural feature is attached to the core polysaccharide and is more elaborate, containing up to 40 repeating subunits of 3 to 5 sugars, referred to as the O antigen, O side chain or O polysaccharide. Variations in the composition of the O antigen account for the species specific antibody responses. Lipid A, acting alone or as a component of LPS, is a potent modulator of the mammalian immune response. The presence of Lipid A or LPS in mammalian macrophages or endothelial cells triggers a signaling cascade leading to the secretion of pro-inflammatory cytokines and nitric oxide. LPS also acts as a B cell mitogen. Differences in Lipid A fatty acid side chain structures may be responsible for variations of the known effects on the immune system.
Lipopolysaccharide from E. coli O111:B4.
Endotoxin:8.2EU/mg (LAL)
Analysis:2-Keto-3-deoxyoctonate (KDO): 2.6% Nucleic acid: 0.6% Protein by Colloidal Gold Transfer Blot: <2.5ng/ug
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute with sterile buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：E. coli O111:B4
Grade：Highly Purified
Purity：Purified
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with 1ml of sterile buffer.
