Tetanus toxin is a potent neurotoxin (150kD) produced by the anaerobic bacterium Clostridium tetani. Intact toxin derived from culture supernatants consists of two polypeptide chains connected through an interchain disulfide bond. The larger of the two polypeptides, heavy chain (100kD), contains the toxin's binding and translocation domains. The smaller of the two polypeptides, light chain (50kD), is a protease which cleaves synaptobrevin 2, also known as VAMP 2. Synaptobrevin 2 is a presynaptic, vesicle membrane associated protein that, in the presence of calcium, forms a stable complex with two plasma membrane attached proteins, syntaxin and SNAP25. Complex formation initiates fusion of transmitter containing vesicles with the plasma membrane. If synaptobrevin 2 is cleaved by tetanus toxin, fusion is prevented and neurotransmitter release is inhibited. Tetanus toxin preferentially blocks release of the inhibitory transmitters, glycine and GABA, when the toxin reaches the inhibitory neurons.
Source:Recombinant protein substrate corresponding to C. botulinum GST-Synaptobrevin-2, 331aa, expressed in E. coli.
Molecular Weight:~37.8kD
Biological Activity:GST-Synaptobrevin-2 can be used as a substrate to assess the activity of Tetanus toxin and Botulinum neurotoxins type B, 0, F and G. Cleavage of GST Synaptobrivin-2 (5uM) is detected with 20nM neurotoxin light chain after incubation at 37°C for 1 hour in 20mM Tris-HCI, pH 8.0, 50mM NaCI.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：~90% (Densitometry analysis)
Form：Supplied as a lyophilized powder in 20mM HEPES, pH 7.4, 1.25% lactose. Reconstitute with 255ul sterile ddH2O.
