Nerve growth factor (NGF) receptor, also known as p75NTR, is a low affinity NGF receptor. It binds with equal affinity neurotrophins such as beta NGF, BDNF, NT-3 and NT-4. NGF receptors mediate signaling of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. These receptors are also thought to play a role in neurogenerative diseases such as Alzheimers disease. The NGF receptor is a type I transmembrane glycoprotein (399aa) consisting of a signal peptide (28aa), an extracellular domain (222aa) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (22aa) and a cytoplasmic domain (155aa).
Source:Nerve Growth Factor Receptor-Fc Chimera, from human DNA sequence encoding the signal peptide and extracellular domain of human NGF receptor aa1-250 was fused to the Fc region of human IgG1 aa93-330. The chimeric protein was expressed in modified human 293 cells.
Molecular Weight:~75kD-100kD with post-translational modification and ~50.8kD unmodified.
Biological Activity:The ED50 of the NGF Receptor-Fc chimera is typically 0.7-1ug/ml as measured by its ability to neutralize beta NGF mediated proliferation of the human growth- factor dependent TF-1 cell line.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Human 293 cells
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder. Reconstitute with 500ul PBS.
