Epidermal Growth Factor (EGF) Receptor binds to EGF and other members of the EGF family such as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. 4 isoforms are produced by alternative splicing (reference SWISSPROT). The EGF receptor is a single-pass type I membrane protein containing an extracellular receptor domain, a transmembrane region and an intracellular domain with tyrosine kinase function.
Source:Epidermal Growth Factor Receptor-Fc Chimera, from human DNA sequence encoding the signal peptide and extracellular domain of human Epidermal Growth Factor Receptor aa1-525 was fused to the Fc region of human IgG1 aa90-330. The chimeric protein was expressed in modified human 293 cells.
Molecular Weight:~85kD-110kD with post-translation modification and ~83kD unmodified.
Biological Activity:The ED50 of the EGF receptor-Fc chimera is typically 60-100ng/ml as measured by its ability to neutralize EGF mediated proliferation of mouse NIH3T3 fribroblasts.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Human 293 cells
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder. Reconstitute with 500ul PBS.
