Wnt-1, formerly known as int-1, is a non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer. Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumor virus (MMTV). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression.
Source:Recombinant protein corresponding to human WNT-1, expressed in E. coli.
Molecular Weight:~38.4kD (343aa residues).
Biological Activity:The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5-2.5ng/ml in the presence of 200ng/ml of human BMP-2.
Applications:Suitable for use in Functional Assays. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Endotoxin: <1EU/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with 100ul sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder in PBS. No preservative added. Reconstitute with 100ul sterile dH2O.
