Murine Vascular Endothelial Growth Factor (VEGF) is a homodimeric protein, where each subunit is 165 amino acids in length. Murine VEGF is expressed by various vascularised tissues and is reported to stimulate endothelial cell growth and angiogenesis.
Source:Recombinant protein corresponding to mouse VEGF expressed in E. coli.
Molecular Weight:~39kD (homodimer of 165aa sequence)
Endotoxin:<0.1ng/ug
Applications:Suitable for use in ELISA, Western Blot and Functional Assays. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3.0ng/laneFunctional Assays: 1-5ng/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with 100ul sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder in 0.1% trifluoroacetic acid. No preservative added. Reconstitute with 100ul sterile dH2O.
