TNF Alpha is an E. coli-derived recombinant protein corresponding to the C-terminal domain of human tumor necrosis factor alpha (TNFa), a pro-inflammatory cytokine with cytotoxic effects on various tumor cells.
Source:Recombinant protein corresponding to 157aa from human TNF Alpha expressed in E. coli.
Molecular Weight:~17.4kD (157aa sequence)
Specific Activity:>2x10e7units/mg as determined by cytolysis of L929 cells in the presence of Actinomycin D.
Endotoxin:<0.1ng/ug
Applications:Suitable for use in ELISA, Western Blot and Functional Assays. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3.0ng/laneFunctional Assays: 0.05-20ng/ml, for functional studies do not add sodium azideOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with 100ul sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder in TBS. No preservative added. Reconstitute with 100ul sterile dH2O. For long term storage the addition of 0.09% sodium azide is recommended.
