Transforming growth factor beta-1 (TGF beta 1) is a homodimeric protein that regulates the proliferation and differentiation of normal and transformed cells. TGF beta 1 is the most abundant isoform which is secreted by most cell types.
Source:Recombinant protein corresponding to human TGF beta 1, expressed in CHO cells.
Molecular Weight:~25.0kD
Biological Activity:The ED50, determined by the ability of TGF beta 1 to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is 0.05ng/ml, corresponding to a specific activity of 2x10e7 units/mg.
Endotoxin:<0.1ng/ug
Applications:Suitable for use in Functional Assays. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with 100ul sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, CHO cells
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul sterile dH2O. Further dilutions should be made in buffer containing a carrier protein such as 0.1% BSA.
