SFRP1 (Secreted Frizzled-Related Protein-1) is a widely expressed multi-functional secreted protein, and member of the sFRP family, which act as soluble modulators of Wnt signalling, playing an important role in embryonic development, and in proliferation, differentiation, and apoptosis in adult tissues. SFRP1 acts as a promoter of Wnt-induced effects at lower concentrations, and as a negative regulator at high concentrations, and has been implicated as a tumor suppressor protein in several human malignancies, including colorectal, breast, bladder, and ovarian cancer.
Source:Recombinant protein corresponding to human SFRP1, expressed in HeLa cells.
Molecular Weight:~283kD
Biological Activity:Determined by its ability to inhibit BMP-2 proliferation effect of ATDC-5 cells, with an expected ED50 of 0.3-0.5ug/ml.
Applications:Suitable for use in Functional Assays. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Endotoxin: <1EU/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with 200ul sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, HeLa cells
Grade：Highly Purified
Purity：~97% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with 200ul sterile dH2O.
