Human resistin, a disulfide-linked homodimeric protein otherwise known as adipose tissue-specific secretory factor, is an adipose-derived hormone and member of the resistin/FIZZ family, implicated as a suppressor of the ability of insulin to reduce blood sugar, and to play a role in type 2 diabetes and obesity. Studies have shown that resistin is involved in an increase in the transcription/expression of many pro-inflammatory cytokines, including interleukin-1,-6 and -12, and also upregulates vascular cell-adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), and monocyte chemotactic protein-1 (MCP-1/CCL2).
Source:Recombinant protein corresponding to human Resistin expressed in E. coli.
Molecular Weight:~19.5kD
Biological Activity:Determined by its ability to stimulate lipolysis in cultured human adipocytes.
Applications:Suitable for use in ELISA, Western Blot and Functional Assays. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3.0ng/laneOptimal dilutions to be determined by the researcher.
Endotoxin: <1EU/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with 250ul ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：25ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder in 10mM sodium citrate. Reconstitute with 250ul sterile dH2O.
