Platelet derived growth factor (PDGF) is a potent mitogen, acting on cell types such as fibroblasts and smooth muscle cells.PDGF is a dimeric protein being composed of A and B chains. The dimer may be formed from AA, BB or AB chain combinations.
Source:Recombinant protein corresponding to human PDGF AA Homodimer, expressed in E. coli.
Molecular Weight:~26.5kD (250aa sequence)
Endotoxin:<0.1ng/mg
Applications:Suitable for use in Functional Assays. Other applications not tested.
Recommended Dilution:Functional Assays: 0.2-10ng/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with 10ul of 10mM acetic acid. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.