PDGF BB Homodimer is an E. coli-derived recombinant protein corresponding to mature full-length human Platelet-Derived Growth Factor BB homodimer (PDGF-BB), a potent mitogen for cells of mesenchymal origin. PDGF is composed of a dimer of two chains, known as the A chain and B chain. The dimer may present as either AA or BB homodimer, or as an AB heterodimer.
Source:Recombinant protein corresponding to human PDGF BB Homodimer, expressed in E. coli.
Molecular Weight:~24.3kD (218aa sequence)
Biological Activity:PDGFB has an ED50 of 1-3ng/ml as determined by stimulation of the proliferation of Balb/c 3T3 cells.
Endotoxin:<0.1ng/ug
Applications:Suitable for use in ELISA, Western Blot and Functional Assays. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3.0ng/laneFunctional Assays: 0.2ng/ml-10ng/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Reconstitute with 100ul sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder in 10mM acetic acid. No preservative added. Reconstitute with 100ul sterile dH2O.
