MMP-3 (matrix metalloproteinase 3), otherwise known as stromelysin-1, is a member of a family of secreted zinc-dependent endoproteases, which play a role in the degradation of extracellular matrix (ECM) components, including both fibrillar and non-fibrillar collagens, laminin, basement membrane glycoprotein and fibronectin. MMP-3 can degrade gelatins type I, III, IV, and V, type III, IV, IX and X collagens, as well as fibronectin, laminin, and cartilage proteoglycans. MMP-3 is implicated in wound repair, and tumor initiation, and studies have shown high expression of MMP-3 by monocytes, and that MMP-3 is also expressed by microglial cells, chondrocytes, and by fibroblast-like synoviocytes in patients with rheumatoid arthritis (RA) and osteoarthritis.
Source:Recombinant protein corresponding to human MMP-3 expressed in E. coli.
Molecular Weight:~42.8kD (378aa) containing the entire catalytic N-terminal domain and the C-terminal domain.
Activity:MMP-3 activity was measured by its ability to cleave a chromogenic peptide MMP-3 substrate at RT. At a MMP-3 concentration of ~2.5ug/ml, 50% cleavage was achieved at an incubation time of ~75 mins.
Applications:Suitable for use in Functional Assays. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Endotoxin Level: <1EU/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder in calcium chloride, CHAPS, sodium phosphate. No preservative added. Reconstitute with 100ul sterile ddH2O.
