Murine macrophage inflammatory protein-1 alpha (MIP-1 alpha), also known as CCL3, is a member of the chemokine family (C-C) and plays an important role in the inflammatory response of blood monocytes and tissue macrophages.
Source:Recombinant protein corresponding to mouse MIP-1 alpha prepared from E. coli.
Molecular Weight:~7.8kD (69aa)
Applications:Suitable for use in ELISA, Western Blot, Functional Assays. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3ng/laneFunctional Assays: 10-100ng/mlOptimal dilutions to be determined by the researcher.
Endotoxin Level: <0.1ng/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:10ug
Source Antigen:Recombinant, E. coli
Grade:Highly Purified
Purity:~98% (SDS-PAGE, HPLC)
Form:Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul sterile ddH2O. For long term storage the addition of 0.1% BSA is recommended.