M-CSF (Macrophage colony-stimulating factor) is a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells. Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor).A genetic defect in M-CSF is responsible for osteopetrosis in mice (op/op), which are severely deficient in mature macrophages and have a restricted capacity for bone remodelling.
Source:Recombinant protein corresponding to mouse M-CSF expressed in E. coli.
Molecular Weight:~36.4kD (156aa)
Activity:The ED50 was determined by the dose dependent stimulation of the proliferation of murine M-NSF-60 cells is <1ng/ml, corresponding to a specific activity of >1x10e6units/mg.
Applications:Suitable for use in Functional Assays. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Endotoxin Level: <1EU/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul sterile ddH2O.
