Recombinant human IL-7 is ~17.4kD protein comprised of 153aa corresponing to the ful-length mature sequence. IL-7 is a potent lymphoid cell growth factor acting on Pre-B, Pro-B and early T cells.
Source:Recombinant protein corresponding to human Interleukin-7 expressed in E. coli.
Molecular Weight:~17.4kD (153aa)
Biological Activity:In a dose dependent cell proliferation assay using 2E8 cells this product shows a specific activity of >2x10e6 units/mg.
Applications:Suitable for use in ELISA, Western Blot, Functional Assays. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3ng/laneFunctional Assays: 0.1-10ng/mlOptimal dilutions to be determined by the researcher.
Endotoxin Level: <0.1ng/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder in 10mM acetic acid. No preservative added. Reconstitute with 100ul sterile ddH2O. For extended storage, the addition of 0.1% BSA is recommended.
