This product, recombinant human interleukin-4, also known as B-cell stimulatory factor 1, is a pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. Excessive secretion of IL-4 by Th2 helper cells, has been linked with allergy and elevated IgE levels.
Source:Recombinant protein corresponding to human Interleukin-4 expressed in E. coli.
Molecular Weight:~14.9kD (129aa)
Biological Activity:The ED50 as determined by the dosedependent stimulation of human TF1 cells is <0.2ng/ml, corresponding to a specific activity of >5x10e6units/mg.
Applications:Suitable for use in ELISA, Western Blot, Functional Assays. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3ng/laneFunctional Assays: 0.1-10ng/mlOptimal dilutions to be determined by the researcher.
Endotoxin Level: <1EU/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：5ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with 50ul sterile ddH2O. For extended storage it is recommended to further dilute in a buffer containing 0.1% BSA.
