Interleukin-1 is a 161aa recombinant IL-10 cytokine, with a range of reported properties including inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cells response. Recombinant human IL-10 has an ED50 of <2.0ng/ml as determined by co-stimulation of MC/9 cells with human IL-4.
Source:Recombinant protein corresponding to human Interleukin-10 expressed in E. coli.
Molecular Weight:~18.6kD
Biological Activity:~5x10e5units/mg
Applications:Suitable for use in ELISA, Western Blot, Functional Assays. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3ng/laneFunctional Assays: 0.2-20ng/mlOptimal dilutions to be determined by the researcher.
Endotoxin Level: <0.1ng/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with 100ul of 5mM sodium phosphate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder in sodium phosphate. No preservative added. Reconstitute with 100ul of 5mM sodium phosphate, pH 7.2.
