Interleukin-1 beta (IL-1 beta), is an ~17.5kD proinflammatory, cell-associated cytokine, produced by monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1 beta promotes the proliferation of memory T cells and cytokine production. It attenuates regulatory T cell function and enables CD4+CD25- autoreactive effectors to avoid suppression. IL-1 beta signals downstream of myc and is required for angiogenesis and invasiveness of tumor cells. IL-1 beta is directly involved in neuronal injury in neurodegenerative disorders, and stimulates mitogenic FGF-like activity, bone resorption and promotes the release of collagenase and prostaglandin from synovial cells.
Source:Recombinant protein corresponding to mouse Interleukin-1 beta expressed in E. coli.
Molecular Weight:~17.5kD (153aa)
Biological Activity:The ED50 as determined by the dose-dependent stimulation of murine D10S cells is <0.002ng/ml, corresponding to a specific activity of >5x10e8units/mg.
Applications:Suitable for use in ELISA, Western Blot, Functional Assays. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3ng/laneOptimal dilutions to be determined by the researcher.
Endotoxin Level: <0.1ng/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul sterile ddH2O.
