GM-CSF (granulocyte-macrophage colony-stimulating factor) is a haematopoietic growth factor which exists in both glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The ability of recombinant GM-CSF to increase haematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation.
Source:Recombinant protein corresponding to human GM-CSF expressed in E. coli.
Molecular Weight:~14.6kD
Applications:Suitable for use in ELISA, Western Blot, Functional Assays. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3.0ng/lane Functional Assays: 1.0ng/mlOptimal dilutions to be determined by the researcher.
Endotoxin: <1EU/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder in 10mM sodium citrate, pH 3.5. Reconstitute with 200ul sterile dH2O.
