Recombinant human epidermal growth factor is 6.2kD globular protein comprosed of 53aa. EGF is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells.
Source:Recombinant protein corresponding to human EGF expressed in E. coli.
Molecular Weight:~6.2kD
Applications:Suitable for use in ELISA, Western Blot and Functional Assays. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3.0ng/laneFunctional Assays: 0.5-25ng/mlOptimal dilutions to be determined by the researcher.
Endotoxin: <0.1 ng/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:500ug
Source Antigen:Recombinant, E. coli
Grade:Highly Purified
Purity:~98% (SDS-PAGE, HPLC)
Form:Supplied as a lyophilized powder. No preservative added. Reconstitute with 0.5ml sterile dH2O.