CD54, otherwise known as ICAM-1, is a widely expressed type I integral membrane glycoprotein and member of the immunoglobulin superfamily, which acts as a ligand for the leukocyte integrin LFA-1 (CD11a/CD18) and Mac-1 integrins, and is the major receptor for human rhinovirus. CD54 is highly expressed on activated endothelial cells and plays a role in transendothelial cell migration to sites of acute inflammation, functioning primarily as an adhesion molecule between endothelial cells and leucocytes, following injury or stress. Levels of soluble CD54 in serum, can be potentially used as an indication of infection, inflammation and certain tumors, and as a target for immunosuppression in transplantation.
Source:Recombinant protein corresponding to CD54 expressed in CHO cells.
Molecular Weight:~49.5kD
Applications:Suitable for use in ELISA, Western Blot, Functional Assays. Other applications not tested.
Recommended Dilution:ELISA: 0.2-0.4ng/wellWestern Blot: 1.5-3.0ng/laneOptimal dilutions to be determined by the researcher.
Endotoxin: <1EU/ug
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, CHO cells
Grade：Highly Purified
Purity：~98% (SDS-PAGE, HPLC)
Form：Supplied as a lyophilized powder in 10mM Sodium Phosphate. No preservative added. Reconstitute with 0.5ml sterile dH2O.
