West Nile virus (WNV) is a virus of the family Flaviviridae part of the Japanese encephalitis (JE) antigenic complex of viruses. Image reconstructions and cryoelectron microscopy reveal a 45-50nm virion covered with a relatively smooth protein surface. This structure is similar to virus; both belong to the genus flavivirus within the family Flaviviridae. WNV is a positive-sense, single strand of RNA, it is between 11,000 and 12,000 nucleotides long which encode seven non-structural proteins and three structural proteins. The RNA strand is held within a nucleocapsid formed from 12kD protein blocks; the capsid is contained within a host-derived membrane altered by two viral glycoproteins.
Source:Recombinant protein corresponding to theWest-Nile N-Terminal Pre-M Virus immunodominant regions, fused to 6x his-tag, expressed in E. coli.
Molecular Weight:~20kD
Applications:Suitable for use in ELISA, Western Blot and as an Antigen for detection of West-Nile virus with minimal specificity problems. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (10% PAGE, coomassie staining).
Form：Supplied as a liquid in 20mM phosphate, pH 7.5.
