85-2068-82　［取扱停止］West Nile Virus, Envelope, Recombinant, His-Tag (WNV) 50ug　209304

特徴

• West Nile virus (WNV) is a virus of the family Flaviviridae part of the Japanese encephalitis (JE) antigenic complex of viruses. Image reconstructions and cryoelectron microscopy reveal a 45-50nm virion covered with a relatively smooth protein surface. This structure is similar to virus; both belong to the genus flavivirus within the family Flaviviridae. WNV is a positive-sense, single strand of RNA, it is between 11,000 and 12,000 nucleotides long which encode seven non-structural proteins and three structural proteins. The RNA strand is held within a nucleocapsid formed from 12kD protein blocks; the capsid is contained within a host-derived membrane altered by two viral glycoproteins.
• Source:Recombinant protein corresponding to West-Nile N-Terminal envelope Virus immunodominant region, fused to His-tag, expressed in E. coli.
• Specificity:Immunoreactive with sera of West-Nile virus infected individuals by ELISA.
• Molecular Weight:~42kD
• AA Sequence:MQLKGTTYGV PCKVPISSVA LEPPFGDSYI ALEMQLKGTT TDGPCKVPIS SVATANAKVL NHHWHKSGSS FLGTPADTGH PVGRLVTVNP QQINHHWHKS CSKAFKFLGT PADTGHGTVV SLNDLTPVGR LVTVNPFVSV VVGRGEQQIN HHWHKSGSSI YGVCSKAFKF LGTPADTGHGSVASLNDLTP IELEPPFGDS IGKAFTTTLK GALEMQLKGT GTVVLELQYT GTDGPCKVPI FVSVATANAK VLIELEPPFG LELQYTGTDG ATANAKVLIE GKAFTTTLKG TVVLELQYTG VGRLVTVNPFV YIVVGRGEQQI TYGVCSKAFK SSVASLNDLT DSYIVVGRGE GSSIGKAFTT TLKGALEHHH HHH
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.