Synaptobrevin 2 is an 18kD integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein (residues 1-89) forms a specific SNARE complex with the target membrane- associated t- or Q-SNAREs syntaxin 1 and SNAP-25.
Source:Recombinant protein corresponding to human VAMP 2, a single, non-glycosylated polypeptide chain containing 126aa (aa1-89), fused to His-tag at N-terminal, expressed in E. coli.
Molecular Weight:~13.8kD
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
