Uracil DNA Glycosylase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 fewer bases). UNG is active over a broad pH range with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.
Source:Uracil DNA Glycosylase, from the E. Coli strain that carries the UNG gene
Applications:Suitable for Glycosylase mediated single nucleotide polymorphism detection (GMPD), site-directed mutagenesis, as a probe for protein-DNA interaction sudies, for rapid and efficient cloning of PCR products and to eliminate carry-over contamination in PCR.
10X UNG Reaction Buffer (Included):Supplied as a liquid in 200mM Tris-HCl, pH 8.0 at 25°C, 10mM DTT, 10mM EDTA.
Unit Definition:1 Unit is defined as the amount of UDG enzyme that catalyzes release of 60pmol of Uracil/minute from double- stranded, uracil-containing DNA in a total reaction volume of 50ul in 30 minutes at 37°C in 1X Uracil DNA Glycosilase Reaction Buffer with 1 Unit of Uracil DNA Glycosylase and 0.2ug [3H]-Uracil DNA (10e4-10e5cpm/ug).
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：2000 IU
Source Antigen：E. coli strain that carries the UNG gene
Grade：Molecular Biology Grade
Purity：Molecular Biology Grade
Form：Supplied as a liquid in 10mM Tris-HCl, pH 7.4 at 25°C, 50mM potassium chloride, 1mM DTT, 0.1mM EDTA, 0.1mg/ml BSA, 50% glycerol.
