85-2068-44　Tropomyosin, Human, Cardiac 25ug　209266

特徴

• Tropomyosin is an actin-binding protein that regulates actin mechanics. It is important, among other things, for muscle contraction. Tropomyosin, along with the troponin complex, associate with actin in muscle fibers and regulate muscle contraction by regulating the binding of myosin. In resting muscle, tropomyosin overlays the myosin binding sites on actin and is "locked" down in this position by troponin T (tropomyosin binding troponin) and troponin I (inhibitory troponin). Upon release of calcium from the sarcoplasmic reticulum calcium binds to troponin C (calcium binding troponin). This "unlocks" tropomyosin from actin, allowing it to move away from the binding groove. Myosin heads can now access the binding sites on actin. Once one myosin head binds, this fully displaces tropomyosin and allows additional myosin heads to bind, initiating muscle shortening and contraction. Once calcium is pumped out of the cytoplasm and calcium levels return to normal, tropomyosin again binds to actin, preventing myosin from binding.
• Tropomyosin is composed of four alpha helices, A, B, C, and D, which coil together to form the quaternary structure. Tropomyosin is a pan-allergen (an allergen widely distributed in nature) because it is a highly conserved protein among species. Certain tropomyosins are known to cause allergies in certain people, and those who have cross-reactive allergies can get symptoms from a range of sources due to a common allergen found in all these sources: Shrimp, dust mites and mollusks. This common allergen is the reason why some people sensitized with mite tropomyosin could have an allergic reaction after eating seafood.
• Source:Tropomyosin, from human cardiac tissue
• Molecular Weight:~32kD (monomer)
• Applications:Suitable for use in ELISA. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.