Treponema pallidum is a gram-negative spirochaete bacterium and is considered to be metabolically crippled. There are at least four known subspecies: T. pallidum pallidum, T. pallidum pertenue, T. pallidum carateum and T. pallidum endemicum. The helical structure of T. pallidum pallidum allows it to move in a corkscrew motion through viscous mediums such as mucus. Treponema pallidum sub sp. pallidum has one of the smallest bacterial genomes at 1.14 million base pairs (Mb) and has limited metabolic capabilities, reflecting its adaptationthrough genome reduction to the rich environment of mammalian tissue.
Source:Recombinant protein corresponding to T. Pallidum p15 immunodominant regions, fused to 6x His-tag at C-terminal, expressed in E. coli.
Specificity:Immunoreactive with sera of T. Pallidum infected individuals.
Molecular Weight:~48kD (multimer)
Applications:Suitable for use in ELISA, Western Blot and as an Antigen for the detection of T. Pallidum with minimal specificity problems. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a liquid in 70mM Tris-HCl, pH 8.0, 50mM sodium chloride, 1.5M urea, 50% glycerol.
